PhD-student to study transcription stress in neurons36 hoursResearch universityResearch & EducationNew

All vacancies PhD-student to study transcription stress in neurons Apply All vacancies New PhD-student to study transcription stress in neurons Investigating how mechanical stress affects transcription-coupled DNA repair in differentiated human neurons Closing date 13-09-2026 3686 36 hours Research university Research & Education Apply Closing date 13-09-2026 3686

Correct gene transcription by RNA polymerase II to transcribe new RNA molecules is crucial for proper cell function. However, transcription by RNA polymerases can be blocked by DNA damage, for example by UV-light or other chemicals. This transcription-blocking DNA damage can therefore cause cellular dysfunction and cell death, eventually resulting in DNA damage-induced aging and genome instability. Cells counteract these deleterious effects by transcription-coupled repair (TCR), which specifically removes DNA damage in our genes, thereby safeguarding transcription. Our lab has identified several important factors that provided novel insights in this repair pathway (van Sluis et al., Nature Cell Biology 2024; Ramadhin et al., Molecular Cell 2024; van Toorn et al., Molecular Cell, 2022; Geijer et al., Nature Cell Biology, 2021; Tresini et al. Nature, 2015; Schwertman et al. Nature Genetics, 2012). In this project, we will investigate how mechanical stress affects transcription-coupled repair and transcription stress in differentiated human neurons. Neurons are long-lived, non-dividing cells that depend strongly on continuous and accurate transcription, but they are also exposed to changes in cellular and nuclear mechanics that may interfere with genome stability. Using iPSC-derived neurons, CRISPR-Cas9 gene editing and advanced live-cell imaging, we will investigate how mechanical stress influences transcription stress and transcription-coupled repair, and how defects in these pathways alter the mechanical properties and stress responses of neurons. This project will provide new mechanistic insight into the interplay between mechanical stress, transcription integrity and DNA repair, and may help explain why differentiated neurons are particularly vulnerable during aging and neurodegenerative disease. Work environment Work environment The department of Molecular Genetics at the Erasmus MC ( ) has a strong world-wide reputation in understanding the DNA damage responses at the molecular, cellular and physiological level. The department consists of a vibrant and international group of more than 80 researchers working on various aspects of DNA damage, DNA repair and other DNA damage-related responses. This project will be carried out in the Marteijn lab ( www.genomestability.nl ) at the Erasmus MC within the Marie Skłodowska-Curie Doctoral Network NEUROMECH ( ), an international consortium investigating the role of mechanobiology in neurodegenerative diseases. The Marteijn lab studies the molecular mechanisms how cells deal with DNA damage that interferes with transcription, using a multidisciplinary approach using molecular and cell biological approaches. Our lab works in a highly collaborative manner with a strong focus on state-of-the-art live-cell imaging, genetic and proteomic tools. Our group acts internationally at the forefront of DNA damage and transcription stress. We offer a dynamic, challenging, and cooperative research environment and an excellent training and supervision platform through the PhD teaching program of our Erasmus MC graduate school ( ). The Erasmus MC stands for a healthy population and excellence in healthcare. By conducting groundbreaking work, we aim to push boundaries through leading the way in research, education and healthcare to improve and renew the healthcare of today and the public health of tomorrow. To stimulate research, the Erasmus MC provides access to the latest equipment and techniques in a state-of-the-art environment. Qualifications and skills Qualifications and skills We are looking for a highly motivated PhD candidate candidate to join the Marie Skłodowska-Curie Doctoral Network NEUROMECH, an international consortium investigating the role of mechanobiology in neurodegenerative diseases. The candidate should have with a MSc. degree in a relevant discipline (e.g. Molecular/Cell biology, Biomedical Sciences, Molecular Sciences, Nanobiology). Experience with the basal transcription process, DNA damage response, Neuronal differentiation systems, live cell imaging is an advantage but not essential. The candidate should be able to independently design, conduct and evaluate research projects, have outstanding theoretical and experimental research skills, is well-organized and result-driven with a strong team spirit. Furthermore, a strong command of (scientific) English in speech and writing is essential (EUR requirements minimum IELTS overall score of 7.0 [comparable to TOEFL PBT: 600; TOEFL CBT: 250; TOEFL IBT: 95]). Being able to present a certificate of good conduct, a valid proof of identity are conditions for the appointment. Applicants must hold (or expect to obtain before recruitment) a Master's degree or equivalent qualification that entitles them to enroll in a doctoral programme. Candidates must not already hold a doctoral degree at the time of recruitment. In accordance with the Marie Skłodowska-Curie Doctoral Network mobility rule, applicants must not have resided or carried out their main activity (work, studies, etc.) in The Netherlands for more than 12 months during the 36 months immediately preceding the recruitment date, irrespective of nationality. Please ensure that you meet the MSCA eligibility criteria before applying. Before you apply please check our conditions for employment . Terms of employment Terms of employment You will receive a temporary position for 4 years. The gross monthly salary is € 3.217,- in the 1st year and gradually increases to € 4.077,- in the 4th year (scale OIO). A salary increase of 3,5% from May 2027, according to the collective labor agreement (CLA). Excellent fringe benefits, such as a 13th month that is already paid out in November and an individual travel expense package. An International Office which aids you in preparing for you arrival and stay. Pension insurance with ABP. We take care of approximately 2/3 of the monthly contribution. Special benefits, such as an incompany physiotherapist and bicycle repairer. And there is also a gym where you can work on your fitness after work. More information More information For more information about this position, please contact Dr. Jurgen Marteijn, phone number: +31 10 7038169 or e-mail: [email protected] . For more information visit www.genomestability.nl . No agencies please. Application proces Application proces 1 Apply Step 1 - Apply Did we get you excited about this position? Submit your application through the application button. You will receive a confirmation of receipt from our recruiter right away. 2 Selection Step 2 - Selection Based on your application, we check to see if there is a fit between us. We will let you know as soon as possible whether you are invited for an interview. 3 Job interview Step 3 - Job interview You have been invited for an interview, great! In this first meeting we get to know each other and see if You can form an idea of the position, the department and Erasmus MC. If the interview goes well usually a second interview follows. 4 Offer and terms of employment Step 4 - Offer and terms of employment It’s a match! Your future manager will discuss your salary and employment with you. You will also receive more information about our other terms of employment. 5 Getting started Step 5 - Getting started Your first working day has come! We are more than happy to have you. Your new department will give you a warm welcome and provide you with all the information you need. Enjoy your job at Erasmus MC! Learn more? Tom Pleij Recruiter +31652905464 Contact via WhatsApp Contact Share vacancy Deel via LinkedIn Share via email Share via WhatsApp Pioneering, pushing boundaries and leading: in healthcare, education and research Erasmus MC is an international leading academic hospital in Rotterdam. We are recognized as a world-class scientific research organization aiming to improve our understanding of diseases and disorders and helps to predict, treat and prevent them. We have access to the latest equipment and techniques. A working environment that gets the best out of people. Find out more about Erasmus MC